Stroke penumbra defined by an MRI-based oxygen challenge technique: 1. validation using [14C]2-deoxyglucose autoradiography

Robertson, C. A., McCabe, C. , Gallagher, L., Lopez-Gonzalez, M. d., Holmes, W. M. , Condon, B., Muir, K. W. , Santosh, C. and Macrae, I. M. (2011) Stroke penumbra defined by an MRI-based oxygen challenge technique: 1. validation using [14C]2-deoxyglucose autoradiography. Journal of Cerebral Blood Flow and Metabolism, 31, pp. 1778-1787. (doi: 10.1038/jcbfm.2011.66)

Abstract

Accurate identification of ischemic penumbra will improve stroke patient selection for reperfusion therapies and clinical trials. Current magnetic resonance imaging (MRI) techniques have limitations and lack validation. Oxygen challenge T2* MRI (T2* OC) uses oxygen as a biotracer to detect tissue metabolism, with penumbra displaying the greatest T2* signal change during OC. [14C]2-deoxyglucose (2-DG) autoradiography was combined with T2* OC to determine metabolic status of T2*-defined penumbra. Permanent middle cerebral artery occlusion was induced in anesthetized male Sprague-Dawley rats (n=6). Ischemic injury and perfusion deficit were determined by diffusion- and perfusion-weighted imaging, respectively. At 147±32 minutes after stroke, T2* signal change was measured during a 5-minute 100% OC, immediately followed by 125 μCi/kg 2-DG, intravenously. Magnetic resonance images were coregistered with the corresponding autoradiograms. Regions of interest were located within ischemic core, T2*-defined penumbra, equivalent contralateral structures, and a region of hyperglycolysis. A T2* signal increase of 9.22%±3.9% (mean±s.d.) was recorded in presumed penumbra, which displayed local cerebral glucose utilization values equivalent to contralateral cortex. T2* signal change was negligible in ischemic core, 3.2%±0.78% in contralateral regions, and 1.41%±0.62% in hyperglycolytic tissue, located outside OC-defined penumbra and within the diffusion abnormality. The results support the utility of OC-MRI to detect viable penumbral tissue followi

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gallagher, Mrs Lindsay and Macrae, Professor Mhairi and Condon, Professor Barrie and Santosh, Dr Celestine and Holmes, Dr William and Muir, Professor Keith and McCabe, Dr Chris
• Authors: Robertson, C. A., McCabe, C., Gallagher, L., Lopez-Gonzalez, M. d., Holmes, W. M., Condon, B., Muir, K. W., Santosh, C., and Macrae, I. M.
• Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
• Journal Name: Journal of Cerebral Blood Flow and Metabolism
• Publisher: Nature
• ISSN: 0271-678X
• Published Online: 11 May 2011
• Copyright Holders: Copyright © 2011 Nature
• First Published: First published in Journal of Cerebral Blood Flow and Metabolism 31:1778-1787
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

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Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
45500	1	Imaging the ischaemic penumbra using BOLD MRI with oxygen challenge as a biotracer	I Macrae	Medical Research Council (MRC)	G0700439	Institute of Neuroscience and Psychology