Carnell, M., Zech, T., Calaminus, S.D., Ura, S., Hagedorn, M., Johnston, S.A., May, R.C., Soldati, T., Machesky, L.M. and Insall, R.H. (2011) Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), pp. 831-839. (doi: 10.1083/jcb.201009119)
Full text not currently available from Enlighten.
Abstract
WASP and SCAR homologue (WASH) is a recently identified and evolutionarily conserved regulator of actin polymerization. In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material. A related process, the expulsion of the lethal endosomal pathogen Cryptococcus neoformans from mammalian macrophages, also uses WASH-coated vesicles, and cells expressing dominant negative WASH mutants inefficiently expel C. neoformans. D. discoideum WASH causes filamentous actin (F-actin) patches to form on lysosomes, leading to the removal of vacuolar adenosine triphosphatase (V-ATPase) and the neutralization of lysosomes to form postlysosomes. Without WASH, no patches or coats are formed, neutral postlysosomes are not seen, and indigestible material such as dextran is not exocytosed. Similar results occur when actin polymerization is blocked with latrunculin. V-ATPases are known to bind avidly-to F-actin. Our data imply a new mechanism, actin-mediated-sorting, in which WASH and the Arp2/3 complex polymerize actin on vesicles to drive the separation and recycling of proteins such as the V-ATPase.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Zech, Mr Tobias and Ura, Mr Seiji and Calaminus, Dr Simon and Carnell, Mr Michael and Machesky, Professor Laura and Insall, Professor Robert |
| Authors: | Carnell, M., Zech, T., Calaminus, S.D., Ura, S., Hagedorn, M., Johnston, S.A., May, R.C., Soldati, T., Machesky, L.M., and Insall, R.H. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Journal of Cell Biology |
| ISSN: | 0021-9525 |
| ISSN (Online): | 1540-8140 |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details