Human papillomavirus 16 E2 stability and transcriptional activation is enhanced by E1 via a direct protein–protein interaction

King, L.E., Dornan, E.S., Donaldson, M.M. and Morgan, I.M. (2011) Human papillomavirus 16 E2 stability and transcriptional activation is enhanced by E1 via a direct protein–protein interaction. Virology, 414(1), pp. 26-33. (doi: 10.1016/j.virol.2011.03.002)

Abstract

Human papillomavirus 16 El and E2 interact with cellular factors to replicate the viral genome. E2 forms homodimers and binds to 12 bp palindromic sequences adjacent to the viral origin and recruits El to the origin. El forms a di-hexameric helicase complex that replicates the viral genome. This manuscript demonstrates that El stabilises the E2 protein, increasing the half life in both C33a and 293 T cells respectively. This stabilisation requires a direct protein-protein interaction. In addition, the El protein enhances E2 transcription function in a manner that suggests the El protein itself can contribute to transcriptional regulation not simply by E2 stabilisation but by direct stimulation of transcription. This activation of E2 transcription is again dependent upon an interaction with El. Overall the results suggest that in the viral life cycle, co-expression of El with E2 can increase E2 stability and enhance E2 function. (C) 2011 Elsevier Inc. All rights reserved

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Dornan, Mr Edward and Morgan, Professor Iain and Donaldson, Dr Mary
• Authors: King, L.E., Dornan, E.S., Donaldson, M.M., and Morgan, I.M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Virology
• Publisher: Elsevier
• ISSN: 0042-6822