Mapping lipid and detergent molecules at the surface of membrane protein

Cogdell, R. J. , Gardiner, A. T. , Roszak, A. W. , Stončius, S. , Kocovsky, P. and Isaacs, N. W. (2011) Mapping lipid and detergent molecules at the surface of membrane protein. Biochemical Society Transactions, 39(3), pp. 775-779. (doi: 10.1042/BST0390775) (PMID:21599648)

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Electron-density maps for the crystal structures of membrane proteins often show features suggesting binding of lipids and/or detergent molecules on the hydrophobic surface, but usually it is difficult to identify the bound molecules. In our studies, heavy-atom-labelled phospholipids and detergents have been used to unequivocally identify these binding sites at the surfaces of test membrane proteins, the reaction centres from Rhodobacter sphaeroides and Blastochloris viridis. The generality of this method is discussed in the present article.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard and Kocovsky, Professor Pavel and Gardiner, Dr Alastair and Isaacs, Professor Neil and Roszak, Dr Aleksander
Authors: Cogdell, R. J., Gardiner, A. T., Roszak, A. W., Stončius, S., Kocovsky, P., and Isaacs, N. W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biochemical Society Transactions
ISSN (Online):1470-8752

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
437241Mapping lipid and detergent binding sites on the surface of membrane proteinsRichard CogdellBiotechnology and Biological Sciences Research Council (BBSRC)BB/E008720/1Institute of Molecular Cell and Systems Biology