Cogdell, R. J. , Gardiner, A. T., Roszak, A. W., Stončius, S., Kocovsky, P. and Isaacs, N. W. (2011) Mapping lipid and detergent molecules at the surface of membrane protein. Biochemical Society Transactions, 39(3), pp. 775-779. (doi: 10.1042/BST0390775) (PMID:21599648)
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Abstract
Electron-density maps for the crystal structures of membrane proteins often show features suggesting binding of lipids and/or detergent molecules on the hydrophobic surface, but usually it is difficult to identify the bound molecules. In our studies, heavy-atom-labelled phospholipids and detergents have been used to unequivocally identify these binding sites at the surfaces of test membrane proteins, the reaction centres from Rhodobacter sphaeroides and Blastochloris viridis. The generality of this method is discussed in the present article.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Roszak, Dr Aleksander and Cogdell, Professor Richard and Gardiner, Dr Alastair and Isaacs, Professor Neil and Kocovsky, Professor Pavel |
| Authors: | Cogdell, R. J., Gardiner, A. T., Roszak, A. W., Stončius, S., Kocovsky, P., and Isaacs, N. W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Biochemical Society Transactions |
| ISSN: | 0300-5127 |
| ISSN (Online): | 1470-8752 |
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