Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression

Ward, R.J., Alvarez-Curto, E. and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746, pp. 21-37. (doi: 10.1007/978-1-61779-126-0_2)

Abstract

The development of a cell-based system that allows the integration of a gene of interest (GOI), such as a G protein-coupled receptor (GPCR), into a specific site on the genome, has made the generation of mammalian cell lines able to express such proteins easy and efficient. Flp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture medium. Stable cell lines generated as described here can be used to great advantage in the study of receptor pharmacology signalling and oligomerisation.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Alvarez-Curto, Dr Elisa and Ward, Dr Richard and Milligan, Professor Graeme
• Authors: Ward, R.J., Alvarez-Curto, E., and Milligan, G.
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
• Journal Name: Methods in Molecular Biology
• ISSN: 1064-3745