Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression

Ward, R.J., Alvarez-Curto, E. and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746, pp. 21-37. (doi: 10.1007/978-1-61779-126-0_2)

Full text not currently available from Enlighten.

Publisher's URL:


The development of a cell-based system that allows the integration of a gene of interest (GOI), such as a G protein-coupled receptor (GPCR), into a specific site on the genome, has made the generation of mammalian cell lines able to express such proteins easy and efficient. Flp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture medium. Stable cell lines generated as described here can be used to great advantage in the study of receptor pharmacology signalling and oligomerisation.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Alvarez-Curto, Dr Elisa and Ward, Dr Richard and Milligan, Professor Graeme
Authors: Ward, R.J., Alvarez-Curto, E., and Milligan, G.
College/School:College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:Methods in Molecular Biology

University Staff: Request a correction | Enlighten Editors: Update this record