Biomimetic microtopography to enhance osteogenesis in vitro

Wilkinson, A., Hewitt, R. N., McNamara, L. E., McCloy, D., Meek, R.M. D. and Dalby, M. J. (2011) Biomimetic microtopography to enhance osteogenesis in vitro. Acta Biomaterialia, 7(7), pp. 2919-2925. (doi: 10.1016/j.actbio.2011.03.026)

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Biomimicry is being used in the next generation of biomaterials. Tuning material surface features such as chemistry, stiffness and topography allow the control of cell adhesion, proliferation, growth and differentiation. Here, microtopographical features with nanoscale depths, similar in scale to osteoclast resorption pits, were used to promote <i>in</i> vitro bone formation in basal medium. Primary human osteoblasts were used to represent an orthopaedically relevant cell type and analysis of adhesions, cytoskeleton, osteospecific proteins (phospho-Runx2 and osteopontin) and mineralisation (alizarin red) was performed. The results further demonstrate the potential for biomimicry in material design and show that the osteoblast response can be tuned from changes in feature size.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and McNamara, Dr Laura and Wilkinson, Dr Andrew and Meek, Professor Dominic
Authors: Wilkinson, A., Hewitt, R. N., McNamara, L. E., McCloy, D., Meek, R.M. D., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Acta Biomaterialia
Published Online:01 April 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
502891Micro- and nano-patterning of titanium surfaces for optimal osseointegration of orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/G048703/1RI MOLECULAR CELL & SYSTEMS BIOLOGY