Multiple paternity in Rhipicephalus (Boophilus) microplus confirmed by microsatellite analysis

Cutullé, C., Jonsson, N.N. and Seddon, J.M. (2010) Multiple paternity in Rhipicephalus (Boophilus) microplus confirmed by microsatellite analysis. Experimental and Applied Acarology, 50(1), pp. 51-58. (doi: 10.1007/s10493-009-9298-3)

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The aim of this study was to determine if individual ticks among the progeny of a single female Rhipicephalus (Boophilus) microplus tick removed from cattle under natural conditions are the result of mating with one or several males. To this end, simulations were run using an existing dataset of genotypes from 8 microsatellite loci to predict the number of samples required and the best locus. Subsequently, 14-22 progeny from each of 15 engorged female ticks removed from three cows, and the engorged females themselves, were genotyped for the BmM1 locus and the minimum number of potential male parents was determined for each progeny group. Of the 15 progeny groups, 10 must have been sired by more than one male, as indicated by the presence of five unique alleles among the progeny or three unique alleles that could not have been contributed by the female. This finding demonstrates multiple paternity in R. microplus.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Jonsson, Professor Nicholas
Authors: Cutullé, C., Jonsson, N.N., and Seddon, J.M.
Subjects:Q Science > QR Microbiology
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Experimental and Applied Acarology
Published Online:20 August 2009

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