Selective Chemical Intervention in the Proteome ofCaenorhabditis elegans

Husi, H. et al. (2010) Selective Chemical Intervention in the Proteome ofCaenorhabditis elegans. Journal of Proteome Research, 9(11), pp. 6060-6070. (doi: 10.1021/pr100427c) (PMID:20804218)

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We present-the first study of protein regulation by ligands in Caenorhabditis elegans. The ligands were peptidyl-prolyl isomerase inhibitors of cyclophilins. Upregulation is observed for several heat shock proteins and one ligand in particular caused a greater than 2-fold enhancement of cyclophilin CYN-5. Additionally, several metabolic enzymes display elevated levels. This approach, using label-free relative quantification, provides an extremely attractive way of measuring the effect of ligands on art entire proteome, with minimal sample pretreatment, which could be applicable to large-scale studies. In this initial study, which compares the effect of three ligands, 54 unique proteins have been identified that are up- (51) or down- (3) regulated in the presence of a given ligand. A total of 431 C. elegans proteins were identified. Our methodology provides an intriguing new direction for in vivo screening of the effects of novel and untested ligands at the whole organism level.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Page, Professor Tony and Butler, Ms Victoria
Authors: Husi, H., McAllister, F., Angelopoulos, N., Butler, V.J., Bailey, K.R., Malone, K., MacKay, L., Taylor, P., Page, A.P., Turner, N.J., Barran, P.E., and Walkinshaw, M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Proteome Research

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
407991Chemical mapping of the PPIase interactome in C elegans - development of a systems biology toolboxAntony PageBiotechnology and Biological Sciences Research Council (BBSRC)BB/D006201/1Infection Immunity and Inflammation Life Sciences