A comprehensive survey of the plasmodium life cycle by genomic, transcriptomic, and proteomic analyses

Hall, N. et al. (2005) A comprehensive survey of the plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. Science, 307(5706), pp. 82-86. (doi: 10.1126/science.1103717)

Abstract

<i>Plasmodium berghei</i> and <i>Plasmodium chabaudi</i> are widely used model malaria species. Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage-specific selective pressures. Four strategies for gene expression are apparent during the parasites' life cycle: (i) housekeeping; (ii) host-related; (iii) strategy-specific related to invasion, asexual replication, and sexual development; and (iv) stage-specific. We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Janssen, Dr Christoph
• Authors: Hall, N., Karras, M., Raine, J.D., Carlton, J.M., Kooij, T.W.A., Berriman, M., Florens, L., Janssen, C.S., Pain, A., Christophides, G.K., James, K., Rutherford, K., Harris, B., Harris, D., Churcher, C., Quail, M.A., Ormond, D., Doggett, J., Trueman, H.E., Mendoza, J., Bidwell, S.L., Rajandream, M.-A., Carucci, D.J., Yates, J.R., Kafatos, F.C., Janse, C.J., Barrell, B., Turner, C.M.R., Waters, A.P., and Sinden, R.E.
• Subjects: Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: Science
• Publisher: American Association for the Advancement of Science
• ISSN: 0036-8075
• ISSN (Online): 1095-9203