Slater, C., Ling, S.C., Preston, T. and Weaver, L.T. (2002) Analysis of C-13-mixed triacylglycerol in stool by bulk (EA- IRMS) and compound specific (GC/MS) methods. Isotopes in Environmental and Health Studies, 38(2), pp. 79-86. (doi: 10.1080/10256010214220)
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Publisher's URL: http://taylorandfrancis.metapress.com/openurl.asp?genre=article&id=doi:10.1080/10256010214220
Abstract
This paper was presented in poster form at the 17th International Congress of Nutrition, August 27-31, Vienna, Austria (Annals of Nutrition and Metabolism 2001; 45(Suppl.1):349). Some of the data were also presented in poster form at the British Society of Gastroenterology Meeting, March 18-21, Glasgow, UK (Gut 2001; 48(Suppl.1):A91). The C-13- mixed triacylglycerol (MTG) breath test is used to measure intraluminal fat digestion. In normal digestion, 20-40% of the ingested C-13 label is recovered in breath CO2. We aimed to identify the proportions of ingested label excreted in stool, as well as breath following ingestion of C-13-MTG by children with impaired exocrine pancreatic function and healthy controls. C-13 enrichment of breath samples was measured by continuous flow isotope ratio mass spectrometry (IRMS) and cumulative percent dose recovered (cPDR) in 10 h was calculated. Total C-13 of a faecal fat extract from each stool was measured by elemental analyser-IRMS, and C-13 enrichment and concentration of the TBDMS derivative of octanoic acid was measured by GC/MS after hydrolysis of the fat extract. Stool 5- day cPDR was calculated. Mean breath cPDR was 35%. Mean cPDR in stool by combustion-IRMS and GC/ MS, respectively, was 0.8% and 1.0%. Therefore, the remaining 64% of the C-13 label must remain in the body and variability in breath cPDR is due to postabsorptive rather than predigestive factors.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Preston, Professor Tom |
Authors: | Slater, C., Ling, S.C., Preston, T., and Weaver, L.T. |
Subjects: | R Medicine > RC Internal medicine |
College/School: | College of Science and Engineering > Scottish Universities Environmental Research Centre |
Journal Name: | Isotopes in Environmental and Health Studies |
ISSN: | 1025-6016 |
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