Pasdeloup, D., Beilstein, F., Roberts, A.P.E., McElwee, M., McNab, D. and Rixon, F.J. (2010) Inner tegument protein pUL37 of herpes simplex virus type 1 is involved in directing capsids to the trans-Golgi network for envelopment. Journal of General Virology, 91(9), pp. 2145-2151. (doi: 10.1099/vir.0.022053-0) (PMID:20505007) (PMCID:PMC3066548)
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Abstract
Secondary envelopment of herpes simplex virus type 1 has been demonstrated as taking place at the trans-Golgi network (TGN). The inner tegument proteins pUL36 and pUL37 and the envelope glycoproteins gD and gE are known to be important for secondary envelopment. We compared the cellular localizations of capsids from a virus mutant lacking the UL37 gene with those of a virus mutant lacking the genes encoding gD and gE. Although wild-type capsids accumulated at the TGN, capsids of the pUL37− mutant were distributed throughout the cytoplasm and showed no association with TGN-derived vesicles. This was in contrast to capsids from a gD−gE− mutant, which accumulated in the vicinity of TGN vesicles, but did not colocalize with them, suggesting that they were transported to the TGN but were unable to undergo envelopment. We conclude that the inner tegument protein pUL37 is required for directing capsids to the TGN, where secondary envelopment occurs.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McNab, Mr David and Rixon, Dr Frazer and McElwee, Dr Marion |
| Authors: | Pasdeloup, D., Beilstein, F., Roberts, A.P.E., McElwee, M., McNab, D., and Rixon, F.J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Research Group: | MRC virology |
| Journal Name: | Journal of General Virology |
| Publisher: | Society for General Microbiology |
| ISSN: | 0022-1317 |
| ISSN (Online): | 1465-2099 |
| Published Online: | 26 May 2010 |
| Copyright Holders: | Copyright © 2010 SGM |
| First Published: | First published in Journal of General Virology 91(2):2145-2151 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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