Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates

Masiga, D.K., Ndung’u, K., Tweedie, A., Tait, A. and Turner, C. (2006) Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates. Experimental Parasitology, 114(3), pp. 149-153. (doi: 10.1016/j.exppara.2006.03.002)

Abstract

We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar (‘type A’) whereas 2 isolates differed substantially (‘type B’). Type A isolates have been generally regarded as genetically identical but AFLP analysis was able to identify multiple differences between them and split the type A T. evansi isolates into two distinct clades.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Masiga, Dr Daniel and Turner, Professor Charles
• Authors: Masiga, D.K., Ndung’u, K., Tweedie, A., Tait, A., and Turner, C.
• Subjects: Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Experimental Parasitology
• Publisher: Elsevier
• ISSN: 0014-4894
• ISSN (Online): 1090-2449
• Copyright Holders: Copyright © 2006 Elsevier
• First Published: First published in Experimental Parasitology 114(3):149-153
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher