Using confocal laser scanning microscopy to image trichome inclusions in amber

Clark, N.D.L. and Daly, C.J. (2010) Using confocal laser scanning microscopy to image trichome inclusions in amber. Journal of Paleontological Techniques, 8, pp. 1-7.

Abstract

Confocal laser scanning microscopy (CLSM) is an analytical technique usually applied to biological and medical samples. It is used to produce high resolution in-focus three dimensional images of thick sections by targeted fluorescence. Trichomes held in amber fluoresce in the far red range whereas amber fluoresces in the ultraviolet. This allows the trichomes to be resolved easily from the amber by CLSM. Samples of amber from two regions were selected for analysis. Baltic amber (Eocene) is well known for its trichome inclusions which have, in the past, been used as a diagnostic feature of that amber. Mexican amber (Middle Miocene) from Simijovel, Chiapas, Mexico also contains abundant trichomes. Samples of amber from both these locations were successfully imaged and re-constructed in 3D using CLSM. This technique enables detailed analysis of the trichome structure without damaging the sample.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Clark, Dr Neil and Daly, Professor Craig
• Authors: Clark, N.D.L., and Daly, C.J.
• College/School: University Services > Library and Collection Services > Museum and Art Gallery; College of Medical Veterinary and Life Sciences > School of Life Sciences
• Journal Name: Journal of Paleontological Techniques
• ISSN: 1646-5806
• Published Online: 01 January 2010