Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation

Haddock, G., Mullin, M., MacCallum, A., Sherry, A. , Tetley, L., Watson, E., Dagleish, M., Smith, D. G.E. and Everest, P. (2010) Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation. Microbiology, 156(10), p. 3079. (doi: 10.1099/mic.0.039867-0) (PMID:20616103)

Abstract

Human small and large intestinal tissue was used to study the interaction of Campylobacter jejuni with its target tissue. The strain used for the study was 81-176 (+pVir). Tissue was processed for scanning and transmission electron microscopy and immunohistochemistry for light microscopy. Organisms adhered to the apical surface of ileal tissues at all time points in large numbers, in areas where mucus was present and in distinct groups. Microcolony formation was evident at 1-2 hours with bacteria adhering to mucus on the tissue surface and to each other by flagella interaction. At later time points (3-4 hours) biofilm formation on ileal tissue was evident. Few organisms were observed in colonic tissue, with organisms present but not as abundant as the ileal tissue. This study shows C. jejuni 81-176 can form microcolonies and biofilms on human intestinal tissue and may be an essential step in its ability to cause diarrhoea in man.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Everest, Dr Paul and Tetley, Dr Laurence and Smith, Professor David and Linn, Dr Aileen and Maccallum, Dr Amanda and Mullin, Mrs Margaret and Dagleish, Dr Mark
• Authors: Haddock, G., Mullin, M., MacCallum, A., Sherry, A., Tetley, L., Watson, E., Dagleish, M., Smith, D. G.E., and Everest, P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Microbiology
• Publisher: Microbiology Society
• ISSN: 1350-0872
• ISSN (Online): 1465-2080