Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

Ohta, A. T., Garcia, M., Valley, J. K., Banie, L., Hsu, H.-Y., Jamshidi, A., Neale, S. L. , Lue, T. and Wu, M. C. (2010) Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers. Lab on a Chip, 10(23), pp. 3213-3217. (doi: 10.1039/c0lc00072h)

Abstract

Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s−1, while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of −0.8 ± 1.0 µm s−1. Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Neale, Professor Steven
• Authors: Ohta, A. T., Garcia, M., Valley, J. K., Banie, L., Hsu, H.-Y., Jamshidi, A., Neale, S. L., Lue, T., and Wu, M. C.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: Lab on a Chip
• Publisher: Royal Society of Chemistry
• ISSN: 1473-0197
• ISSN (Online): 1473-0189
• Published Online: 08 September 2010