Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

Hsu, H.-y., Ohta, A. T., Chiou, P.-Y., Jamshidi, A., Neale, S. L. and Wu, M. C. (2010) Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media. Lab on a Chip, 10(2), pp. 165-172. (doi: 10.1039/b906593h)

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Publisher's URL: http://dx.doi.org/10.1039/b906593h

Abstract

Optoelectronic tweezers (OET), based on light-induced dielectrophoresis, has been shown as a versatile tool for parallel manipulation of micro-particles and cells (P. Y. Chiou, A. T. Ohta and M. C. Wu, Nature, 2005, 436, 370–372).1 However, the conventional OET device cannot operate in cell culture media or other high-conductivity physiological buffers due to the limited photoconductivity of amorphous silicon. In this paper, we report a new phototransistor-based OET (Ph-OET). Consisting of single-crystalline bipolar junction transistors, the Ph-OET has more than 500× higher photoconductivity than amorphous silicon. Efficient cell trapping of live HeLa and Jurkat cells in Phosphate Buffered Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) has been demonstrated using a digital light projector, with a cell transport speed of 33 µm/sec, indicating a force of 14.5 pN. Optical concentration of cells and real-time control of individually addressable cell arrays have also been realized. Precise control of separation between two cells has also been demonstrated. We envision a new platform for single cell studies using Ph-OET.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neale, Professor Steven
Authors: Hsu, H.-y., Ohta, A. T., Chiou, P.-Y., Jamshidi, A., Neale, S. L., and Wu, M. C.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Published Online:05 August 2009

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