Pflock, T., Dezi, M., Venturoli, G., Cogdell, R.J., Köhler, J. and Oellerich, S. (2008) Comparison of the fluorescence kinetics of detergent-solubilized and membrane-reconstituted LH2 complexes from Rps. acidophila and Rb. sphaeroides. Photosynthesis Research, 95(2-3), pp. 291-298. (doi: 10.1007/s11120-007-9245-2) (PMID:17912609)
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Publisher's URL: http://dx.doi.org/10.1007/s11120-007-9245-2
Abstract
Picosecond time-resolved fluorescence spectroscopy has been used in order to compare the fluorescence kinetics of detergent-solubilized and membrane-reconstituted light-harvesting 2 (LH2) complexes from the purple bacteria <i>Rhodopseudomonas</i> (<i>Rps.</i>) <i>acidophila</i> and <i>Rhodobacter</i> (<i>Rb.</i>) <i>sphaeroides</i>. LH2 complexes were reconstituted in phospholipid model membranes at different lipid:protein-ratios and all samples were studied exciting with a wide range of excitation densities. While the detergent-solubilized LH2 complexes from <i>Rps. acidophila</i> showed monoexponential decay kinetics (tf = 980 ps) for excitation densities of up to 3ú1013 photons/(pulseúcm2), the membrane-reconstituted LH2 complexes showed multiexponential kinetics even at low excitation densities and high lipid:protein-ratios. The latter finding indicates an efficient clustering of LH2 complexes in the phospholipid membranes. Similar results were obtained for the LH2 complexes from <i>Rb. sphaeroides</i>.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cogdell, Professor Richard |
| Authors: | Pflock, T., Dezi, M., Venturoli, G., Cogdell, R.J., Köhler, J., and Oellerich, S. |
| Subjects: | Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Photosynthesis Research |
| ISSN: | 0166-8595 |
| Published Online: | 03 October 2007 |
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