Triptolide simultaneously induces reactive oxygen species, inhibits NF-κB activity and sensitizes 5-fluorouracil in colorectal cancer cell lines

Xu, B., Guo, X., Mathew, S., Armesilla, A. L., Cassidy, J., Darling, J. L. and Wang, W. (2010) Triptolide simultaneously induces reactive oxygen species, inhibits NF-κB activity and sensitizes 5-fluorouracil in colorectal cancer cell lines. Cancer Letters, 291(2), pp. 200-208. (doi: 10.1016/j.canlet.2009.10.013)

Abstract

The in vitro chemosensitizing effect of triptolide (PG490) on the cytotoxicity of 5-fluorouracil (5-FU) was determined in three colorectal cancer (CRC) cell lines. PG490 alone was toxic to CRC cell lines (IC(50)s: 1.39-5.51 nM). The cytotoxicity of 5-FU to CRC cell lines was 4-15-fold sensitized by combining use of low concentration of PG490. CI-isobologram indicated that the effect of PG490 plus 5-FU was synergistic. PG490 and 5-FU treatment induced activated caspase 3 and Bax expression and inhibited Bcl-2 expression. PG490 simultaneously induced generation of reactive oxygen species (ROS) and inhibited NF-kappa B transcriptional activity. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cassidy, Professor James
• Authors: Xu, B., Guo, X., Mathew, S., Armesilla, A. L., Cassidy, J., Darling, J. L., and Wang, W.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Cancer Letters
• ISSN: 0304-3835
• Published Online: 01 January 2010