On-chip immunoprecipitation for protein purification

Sandison, M. E., Cumming, S. A., Kolch, W. and Pitt, A. R. (2010) On-chip immunoprecipitation for protein purification. Lab on a Chip, 10(20), pp. 2805-2813. (doi: 10.1039/C005295G) (PMID:20714512)

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Publisher's URL: http://dx.doi.org/10.1039/C005295G


Immunoprecipitation (IP) is one of the most widely used and selective techniques for protein purification. Here, a miniaturised, polymer-supported immunoprecipitation ([small micro]IP) method for the on-chip purification of proteins from complex mixtures is described. A 4 [small micro]l PDMS column functionalised with covalently bound antibodies was created and all critical aspects of the [small micro]IP protocol (antibody immobilisation, blocking of potential non-specific adsorption sites, sample incubation and washing conditions) were assessed and optimised. The optimised [small micro]IP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate

Item Type:Articles
Glasgow Author(s) Enlighten ID:Kolch, Prof Walter and Sandison, Dr Mairi and Pitt, Dr Andrew and Cumming, Dr Sarah
Authors: Sandison, M. E., Cumming, S. A., Kolch, W., and Pitt, A. R.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN (Online):1473-0189
Published Online:17 August 2010

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
387191Radical Solutions for Researching the Proteome (RASOR)Andrew PittBiotechnology and Biological Sciences Research Council (BBSRC)BB/C511572/1Institute of Molecular Cell and Systems Biology