Tailoring Cell Behavior on Polymers by the Incorporation of Titanium Doped Phosphate Glass Filler

Chrzanowski, W., Abou Neel, E.A., Lee, K.Y., Bismarck, A., Young, A.M., Hart, A.D., Dalby, M.J. and Knowles, J.C. (2010) Tailoring Cell Behavior on Polymers by the Incorporation of Titanium Doped Phosphate Glass Filler. Advanced Biomaterials, 12(7), B298-B308. (doi: 10.1002/adem.200980083)

Abstract

Understanding tissue response to materials, to enable modulation and guided tissue regeneration is one of the main challenges in biomaterials science. Nowadays polymers, glasses, and metals dominate as biomaterials. Often native properties of those materials are not sufficient and there is a need to combine them, so as to modify and adjust their properties to the application. The primary aim of this study was to improve cell response to polymer (PLDL) using phosphate glass as filler (titanium doped phosphate glass). As a control beta-tricalcium phosphate (TCP) filler was used. Various concentrations of the filler were used (10-40 vol%). Wetting behavior, zeta-potentials, mechanical and thermal properties, and human cells response to the materials were evaluated. Results showed that with increase in glass filler loading wettability improved, zeta-potentials dropped, and increase in stiffness of materials was observed. Importantly cell culture experiments showed more developed and well spread cells on the samples with glass content up to 20 vol%. Cells responded much more positively to the glass filled samples than to TCP filled. However, expression of osteocalcin and osteopontin, proteins that indicate formation of the mineralized structures was positive for all the samples including pure PLDL. It was concluded that due to improved wetting behavior, lower zeta-potentials, and specific chemistry of the glass filler it was possible to alter cells response, improve bioactivity of the polymer, and vary mechanical properties.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Dalby, Professor Matthew and Chrzanowski, Dr Wojciech and Hart, Mr Andrew
• Authors: Chrzanowski, W., Abou Neel, E.A., Lee, K.Y., Bismarck, A., Young, A.M., Hart, A.D., Dalby, M.J., and Knowles, J.C.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Engineering > Systems Power and Energy
• Journal Name: Advanced Biomaterials
• Publisher: Wiley-VCH
• ISSN: 1438-1656