Lab-on-a-chip technologies for proteomic analysis from isolated cells

Cooper, J.M., Kolch, W., Monaghan, P.B., Caron, F. and Sedgwick, H. (2008) Lab-on-a-chip technologies for proteomic analysis from isolated cells. Journal of the Royal Society: Interface, 5(S2), S123-S130. (doi: 10.1098/rsif.2008.0169)

Abstract

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kolch, Prof Walter
• Authors: Cooper, J.M., Kolch, W., Monaghan, P.B., Caron, F., and Sedgwick, H.
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: Journal of the Royal Society: Interface
• Publisher: The Royal Society
• ISSN: 1742-5689
• ISSN (Online): 1742-5662