Age‐related and infection intensity–related shifts in antibody recognition of defined protein antigens in a schistosome‐exposed population

Mutapi, F., Burchmore, R. , Mduluza, T., Midzi, N., Turner, C. M. R. and Maizels, R. M. (2008) Age‐related and infection intensity–related shifts in antibody recognition of defined protein antigens in a schistosome‐exposed population. Journal of Infectious Diseases, 198(2), pp. 167-175. (doi: 10.1086/589511)

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Publisher's URL: http://dx.doi.org/10.1086/589511

Abstract

<b>Background:</b> This study compared patterns of recognition of defined Schistosoma haematobium adult worm antigens by serum antibodies from schistosome-exposed Zimbabweans aged 5–18 years.<p></p> <b>Methods:</b> The population was stratified by age and infection intensity into 9 groups within which serum specimens were pooled and used to screen for protein recognition by 2-dimensional Western blotting. Recognized proteins were identified by electrospray ionizing tandem mass spectrometry.<p></p> <b>Results:</b> A total of 71 antigens were recognized by ≥1 of the serum pools. The recognition varied distinctly with host age and infection intensity, with some isoform-specific responses. The repertoire of antigens recognized increased with age, peaking in the oldest participants whose had no or mild-to-moderate infection intensity. The intensity of antigen recognition also increased with age, peaking in the oldest participants with the heaviest infection intensity.<p></p> <b>Conclusions:</b> The recognition of specific schistosome antigens, both in terms of the diversity of antigens recognized and the intensity of antigen recognition, increased with duration of exposure to infection, supporting the hypothesis that the slow development of schistosome-acquired immunity is due to the slow accumulation of responsiveness to relevant parasite antigens.<p></p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mutapi, Dr Francisca and Turner, Professor Charles and Burchmore, Dr Richard
Authors: Mutapi, F., Burchmore, R., Mduluza, T., Midzi, N., Turner, C. M. R., and Maizels, R. M.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Infectious Diseases
Journal Abbr.:J Infect Dis.
Publisher:Oxford University Press
ISSN:0022-1899
ISSN (Online):1537-6613
Published Online:19 June 2008
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