Microfluidic array platform for simultaneous lipid bilayer membrane formation

Zagnoni, M., Sandison, M.E. and Morgan, H. (2009) Microfluidic array platform for simultaneous lipid bilayer membrane formation. Biosensors and Bioelectronics, 24(5), pp. 1235-1240. (doi: 10.1016/j.bios.2008.07.022)

Abstract

In recent years, protein array technologies have found widespread applications in proteomics. However, new methods for high-throughput analysis of protein-protein and protein-compound interactions are still required. In this paper, an array of lipid bilayer membranes formed within a microfluidic system with integrated electrodes is presented. The system is comprised of three layers that are clamped together, thus rendering the device cleanable and reusable. The device microfluidics enable the simultaneous formation of an array of lipid bilayers using a previously developed air-exposure technique, thereby avoiding the need to manually form individual bilayers. The Ag/AgCl electrodes allow for ion channel measurements, each of the sites being independently addressable. Typically, a 50% yield in simultaneous lipid bilayer formation over 12 sites was obtained and ion channel recordings have been acquired over multiple sites. This system has great potential for the development of an automatable platform of suspended lipid bilayer arrays.

• Item Type: Articles
• Keywords: APERTURES Arrays CANCER BIOMARKER DISCOVERY channel CHIP CONTROLLED DELIVERY device electrodes Ion channel recording layer LAYERS MEMBRANES MICROARRAYS Microfluidic Microfluidic array microfluidics On-chip lipid bilayer PATCH-CLAMP PROTEIN PROTEINS proteomics RECONSTITUTION recordings si system technologies technology
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Sandison, Dr Mairi and Zagnoni, Dr Michele
• Authors: Zagnoni, M., Sandison, M.E., and Morgan, H.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: Biosensors and Bioelectronics
• ISSN: 0956-5663