Iwanaga, S., Khan, S.M., Kaneko, I., Christodoulou, Z., Newbold, C.N., Yuda, M., Janse, C.J. and Waters, A.P. (2010) Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome. Cell Host and Microbe, 7(3), pp. 245-255. (doi: 10.1016/j.chom.2010.02.010)
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Abstract
The artificial chromosome represents a useful tool for gene transfer, both as cloning vectors and in chromosome biology research. To generate a Plasmodium artificial chromosome (PAC), we had to first functionally identify and characterize the parasite's centromere. A putative centromere (pbcen5) was cloned from chromosome 5 of the rodent parasite P. berghei based on a Plasmodium gene-synteny map. Plasmids containing pbcen5 were stably maintained in parasites during a blood-stage infection with high segregation efficiency, without drug pressure. pbcen5-containing plasmids were also stably maintained during parasite meiosis and mitosis in the mosquito. A linear PAC (L-PAC) was generated by integrating pbcen5 and telomere into a plasmid. The L-PAC segregated with a high efficiency and was stably maintained throughout the parasite's life cycle, as either one or two copies. These results suggest that L-PAC behaves like a Plasmodium chromosome, which can be exploited as an experimental research tool.
Item Type: | Articles (Other) |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Waters, Professor Andy |
Authors: | Iwanaga, S., Khan, S.M., Kaneko, I., Christodoulou, Z., Newbold, C.N., Yuda, M., Janse, C.J., and Waters, A.P. |
Subjects: | Q Science > QR Microbiology |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Cell Host and Microbe |
ISSN: | 1931-3128 |
ISSN (Online): | 1934-6069 |
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