Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography

Dalby, M.J. , Giannaras, D., Riehle, M.O. , Gadegaard, N. , Affrossman, S. and Curtis, A.S.G. (2004) Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography. Biomaterials, 25(1), pp. 77-83. (doi: 10.1016/S0142-9612(03)00475-7)

Abstract

It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27 nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture.

• Item Type: Articles
• Keywords: Nano-topography, fibroblasts, cytoskeleton, cell adhesion.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Curtis, Professor Adam and Gadegaard, Professor Nikolaj and Dalby, Professor Matthew and Riehle, Dr Mathis
• Authors: Dalby, M.J., Giannaras, D., Riehle, M.O., Gadegaard, N., Affrossman, S., and Curtis, A.S.G.
• Subjects: R Medicine > R Medicine (General); T Technology > TP Chemical technology
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; University Centres > Glasgow Materials Research Initiative
• Journal Name: Biomaterials
• ISSN: 0142-9612