Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

McMillan, N., Smith, L.V., de la Fuente, J.M., Parenty, A.D.C., Gadegaard, N. , Pitt, A.R., Thomson, K., MacKenzie, C., Kelly, S. and Cronin, L. (2007) Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification. Chemical Communications, 25, pp. 2581-2583. (doi: 10.1039/b702575k)

Abstract

N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Parenty, Mr Alexis and McMillan, Miss Nicola and Pitt, Dr Andrew and Gadegaard, Professor Nikolaj and Cronin, Professor Lee
• Authors: McMillan, N., Smith, L.V., de la Fuente, J.M., Parenty, A.D.C., Gadegaard, N., Pitt, A.R., Thomson, K., MacKenzie, C., Kelly, S., and Cronin, L.
• Subjects: Q Science > QD Chemistry
• College/School: College of Science and Engineering > School of Chemistry; University Centres > Glasgow Materials Research Initiative
• Journal Name: Chemical Communications
• ISSN: 1359-7345