Accurate determination of microbial diversity from 454 pyrosequencing data

Quince, C., Lanzen, A., Curtis, T.P., Davenport, R.J., Hall, N., Head, I.M., Read, F. and Sloan, W.T. (2009) Accurate determination of microbial diversity from 454 pyrosequencing data. Nature Methods, 6(9), 639-U27. (doi: 10.1038/NMETH.1361) (PMID:19668203)

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Abstract

We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Quince, Dr Christopher and Sloan, Professor William
Authors: Quince, C., Lanzen, A., Curtis, T.P., Davenport, R.J., Hall, N., Head, I.M., Read, F., and Sloan, W.T.
Subjects:T Technology > TA Engineering (General). Civil engineering (General)
College/School:College of Science and Engineering > School of Engineering > Infrastructure and Environment
Journal Name:Nature Methods
ISSN:1548-7091
ISSN (Online):1548-7105
Published Online:09 August 2009

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
405371COMIX coupling of biofilm diversity and ecosystem functioning the role of communication and mixing in microbail landscapesWilliam SloanNatural Environment Research Council (NERC)NE/D522211/1ENG - ENGINEERING INFRASTRUCTURE & ENVIR