Analysis of the spectrotypes of autoantibodies against thyroglobulin in two rat models of autoimmune thyroiditis

Stott, D.I., Hassman, R., Neilson, L. and McGregor, A.M. (1988) Analysis of the spectrotypes of autoantibodies against thyroglobulin in two rat models of autoimmune thyroiditis. Clinical and Experimental Immunology, 73(2), pp. 269-275.

Abstract

We have studied the isoelectric focusing (IEF) spectrotypes of autoantibodies against thyroglobulin in two rat models of Hashimoto's thyroiditis: (1) AUG strain rats immunized with autologous thyroglobulin in Freund's complete adjuvant; (2) PVG/c strain rats which have been thymectomized and sublethally irradiated. The IEF spectrotypes revealed differences between the two models. The anti-thyroglobulin response in immunized AUG rats is mainly oligoclonal or polyclonal, often with dominant clones in the spectrotype, similar to about 90% of Hashimoto's patients, whereas the response in the PVG/c rat is highly restricted. There were pronounced changes in spectrotype with time in the PVG/c, but not AUG, rats with considerable variation in the lifespan of individual clones. The maximum lifespan of an anti-self secreting B-cell clone in the PVG/c rat, determined by persistence of its clonotype, was at least 16 weeks.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Stott I, Professor David
• Authors: Stott, D.I., Hassman, R., Neilson, L., and McGregor, A.M.
• Subjects: Q Science > QR Microbiology > QR180 Immunology
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Clinical and Experimental Immunology
• Publisher: Wiley-Blackwell Publishing Ltd.
• ISSN: 0009-9104