Jones, L. A., Roberts, F., Nickdel, M. B., Brombacher, F., McKenzie, A. N. J., Henriquez, F. L., Alexander, J. and Roberts, C. W. (2010) IL-33 receptor (T1/ST2) signalling is necessary to prevent the development of encephalitis in mice infected with Toxoplasma gondii. European Journal of Immunology, 40(2), pp. 426-436. (doi: 10.1002/eji.200939705)
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Abstract
T1/ST2 is an immunoregulatory protein of the IL-1 receptor family that has recently been reported as being a component of the IL-33 receptor. IL-33 is a newly described cytokine known to amplify the Th2 response and reduce production of Th1 cytokines. The function of T1/ST2 during Toxoplasma gondii infection is as yet undescribed. Given the requirement of a balanced type 1/type 2 response for effective control of parasite number and immunopathology, it is likely that T1/ST2 may play a part in aiding this process. Accordingly, we have shown that T1/ST2 mRNA transcripts are upregulated in the brains of mice infected with T. gondii and that mice deficient in T1/ST2 demonstrated increased susceptibility to infection with T. gondii that correlated with increased pathology and greater parasite burden in the brains. Real-time PCR analysis of cerebral cytokine levels revealed increased mRNA levels of iNOS, IFN-gamma and TNF-alpha in infected T1/ST2(-/-) mice. These effects were independent of changes in IL-10 production. This study provides the first evidence of a specific role for IL-33 receptor signalling in the brain as well as highlighting the requirement of this mechanism in limiting infection with an intracellular parasite
Item Type: | Articles |
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Keywords: | allergy BRAIN CD4(+) T-CELLS Encephalitis FACTOR-ALPHA GAMMA-INTERFERON GENE-EXPRESSION GENETICALLY RESISTANT BALB/C IFN-GAMMA IL-33 IL-33 receptor INFECTION LEVEL MECHANISM NITRIC-OXIDE SYNTHASE NUMBER PROTEIN SUSCEPTIBILITY SUSCEPTIBLE C57BL/6 MICE Th1/Th2 TIME QUANTITATIVE PCR Toxoplasma TUMOR-NECROSIS-FACTOR |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Roberts, Dr Fiona |
Authors: | Jones, L. A., Roberts, F., Nickdel, M. B., Brombacher, F., McKenzie, A. N. J., Henriquez, F. L., Alexander, J., and Roberts, C. W. |
College/School: | College of Medical Veterinary and Life Sciences |
Journal Name: | European Journal of Immunology |
ISSN: | 0014-2980 |
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