Bioavailability of Anthocyanins and Ellagitannins Following Consumption of Raspberries by Healthy Humans and Subjects with an Ileostomy

Gonzalez-Barrio, R., Borges, G., Mullen, W. and Crozier, A. (2010) Bioavailability of Anthocyanins and Ellagitannins Following Consumption of Raspberries by Healthy Humans and Subjects with an Ileostomy. Journal of Agricultural and Food Chemistry, 58(7), pp. 3933-3939. (doi: 10.1021/jf100315d)

Abstract

The fate of anthocyanins, ellagic acid, and ellagitannins was studied following the consumption of 300 g of raspberries by healthy human volunteers and subjects with an ileostomy. Postingestion plasma and urine from the former and ilea l fluid and urine from the latter group were collected and analyzed by HPLC-PDA-MS2. Plasma from the healthy volunteers did not contain detectable quantities of either the native raspberry polyphenolics or their metabolites. The three main raspberry anthocyanins were excreted in urine in both healthy and ileostomy volunteers 0-7 h after ingestion, in quantities corresponding to <0.1% of intake. This indicates a low level of absorption in the small intestine. With ileostomy volunteers 40% of anthocyanins and 23% of the ellagitannin sanguiin H-6 were recovered in ileal fluid with the main excretion period being the first 4 h after raspberry consumption. The recovery of ellagic acid in ileal fluid was 241%, indicating hydrolysis of ellagitannins in the stomach and/or the small intestine. Urinary excretion of ellagic acid and an ellagic acid-O-glucuronide was <1% of intake. No intact or conjugated forms of ellagitannins were detected in urine from either healthy subjects or ileostomy volunteers. However, in healthy subjects, but not the ileostomists, ellagitannins were catabolized with the appearance of urolithin A-O-glucuronide, two of its isomers, and urolithin B-O-glucuronide in urine collected 7-48 h after raspberry consumption. There was marked variation in the urolithin profile of individual volunteers, indicating differences in the colonic microflora responsible for ellagitannin degradation

• Item Type: Articles
• Keywords: ABSORPTION anthocyanins ANTIOXIDANT CAPACITY ATHEROSCLEROSIS BIOAVAILABILITY CANCER colonic metabolites CONSUMPTION DERIVATIVES ellagic acid Ellagitannins FLUID GASTROINTESTINAL-TRACT GREEN TEA FLAVAN-3-OLS HEALTHY HUMANS Human HUMAN PLASMA HUMANS IDENTIFICATION Ileostomists LEVEL Metabolism METABOLITES MICROFLORA PLASMA POMEGRANATE JUICE PROFILE raspberries RECOVERY Scotland SMALL-INTESTINE STOMACH TECHNOLOGIES URINE urolithins
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Borges, Dr Gina and Crozier, Professor Alan and Mullen, Dr Bill and Gonzalez-Barrio, Dr Rocio
• Authors: Gonzalez-Barrio, R., Borges, G., Mullen, W., and Crozier, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences
• Journal Name: Journal of Agricultural and Food Chemistry
• ISSN: 0021-8561