Identification of hepatitis a virus mimotopes by phage display, antigenicity and immunogenicity

Larralde, O.G., Martinez, R., Camacho, F., Amin, N., Aguilar, A., Talavera, A., Stott, D.I. and Perez, E.M. (2007) Identification of hepatitis a virus mimotopes by phage display, antigenicity and immunogenicity. Journal of Virological Methods, 140(1-2), pp. 49-58. (doi: 10.1016/j.jviromet.2006.10.015)

Abstract

A phage-displayed peptide approach was used to identify ligands mimicking antigenic determinants of hepatitis A virus (HAV) for the first time. Bacteriophages displaying HAV mimotopes were isolated from a phage-display peptide library by affinity selection on serum antibodies from hepatitis A patients. Selected phage-peptides were screened for reactivity with sera from HAV infected patients and healthy controls. Four cloned peptides with different sequences were identified as mimotopes of HAV; three of them showed similarity in their amino acid sequences with at least one of the VP3 and VP1 antigenic proteins of HAV. One clone was recognised by 92% of the positive sera. The phagotopes competed effectively with HAV for absorption of anti-HAV-specific antibodies in human sera, as determined by ELISA. The four phage clones induced neutralising anti-HAV antibodies in immunised mice. These results demonstrate the potential of this method to elucidate the disease related epitopes of HAV and to use these mimotopes in diagnostic applications or in the development of a mimotope-based hepatitis A vaccine without the necessity of manipulation of the virus.

• Item Type: Articles
• Keywords: Phage display, HAV, mimotopes, vaccine, diagnostic.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Stott I, Professor David
• Authors: Larralde, O.G., Martinez, R., Camacho, F., Amin, N., Aguilar, A., Talavera, A., Stott, D.I., and Perez, E.M.
• Subjects: Q Science > QR Microbiology > QR180 Immunology
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of Virological Methods
• Publisher: Elsevier
• ISSN: 0166-0934
• Copyright Holders: Copyright © 2007 Elsevier
• First Published: First published in Journal of Virological Methods 140(1-2):49-58
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher.