The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus

Fraser, N.L.W., Rowley, G., Field, M. and Stott, D.I. (2003) The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus. Arthritis Research and Therapy, 5, pp. 114-121. (doi: 10.1186/ar627)




In systemic lupus erythematosus (SLE) it has been hypothesized that self-reactive B cells arise from virgin B cells that express low-affinity, nonpathogenic germline V genes that are cross-reactive for self and microbial antigens, which convert to high-affinity autoantibodies via somatic hypermutation. The aim of the present study was to determine whether the V<sub>H</sub> family repertoire and pattern of somatic hypermutation in germinal centre (GC) B cells deviates from normal in SLE. Rearranged immunoglobulin V<sub>H</sub> genes were cloned and sequenced from GCs of a SLE patient's spleen. From these data the GC V gene repertoire and the pattern of somatic mutation during the proliferation of B-cell clones were determined. The results highlighted a bias in V<sub>H</sub>5 gene family usage, previously unreported in SLE, and under-representation of the V<sub>H</sub>1 family, which is expressed in 20–30% of IgM+ B cells of healthy adults and confirmed a defect in negative selection. This is the first study of the splenic GC response in human SLE.

Item Type:Articles
Keywords:Spleen, systematic lupus erythematosus.
Glasgow Author(s) Enlighten ID:Stott I, Professor David
Authors: Fraser, N.L.W., Rowley, G., Field, M., and Stott, D.I.
Subjects:Q Science > QR Microbiology > QR180 Immunology
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Arthritis Research and Therapy
Publisher:BioMed Central
ISSN (Online):1478-6362
Copyright Holders:Copyright © 2003 BioMed Central
First Published:First published in Arthritis Research and Therapy 5:114-121
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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