Functional genomics of hsp-90 in parasitic and free-living nematodes

Gillan, V. , Maitland, K. , McCormack, G., Him, N. A.I.I. and Devaney, E. (2009) Functional genomics of hsp-90 in parasitic and free-living nematodes. International Journal for Parasitology, 39(10), pp. 1071-1081. (doi: 10.1016/j.ijpara.2009.02.024)

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Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules were functionally characterised by rescue of a Ce-daf-21 (hsp-90) null mutant. Our results show a gradient of rescue: the C elegans endogenous gene provided full rescue of the daf-21 mutant, while Hc-hsp-90 provided partial rescue. In contrast, no rescue could be obtained using a variety of Bp-hsp-90 constructs, despite the fact that Bp-hsp-90 was transcribed and translated in the mutant worms. daf-21 RNA interference (RNAi) experiments were carried out to determine whether knock-down of the endogenous daf-21 mRNA in N2 worms could be complemented by expression of either parasite gene. However neither parasite gene could rescue the daf-21 (RNAi) phenotypes. These results indicate that factors other than the level of sequence identity are important for determining whether parasite genes can functionally complement in C. elegans.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen and Maitland, Ms Kirsty and McCormack, Ms Gillian and Gillan, Dr Victoria
Authors: Gillan, V., Maitland, K., McCormack, G., Him, N. A.I.I., and Devaney, E.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:International Journal for Parasitology
ISSN (Online):1879-0135
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
241291Molecular Genetic and Biochemical Analyses of ParasitesJeremy MottramMedical Research Council (MRC)G97222968Infection Immunity and Inflammation Life Sciences