Marchetti, B., Gault, E.A., Cortese, M.S., Yuan, Z.Q., Ellis, S.A., Nasir, L. and Campo, M.S. (2009) Bovine papillomavirus type 1 oncoprotein E5 inhibits equine MHC class I and interacts with equine MHC I heavy chain. Journal of General Virology, 90(12), pp. 2865-2870. (doi: 10.1099/vir.0.014746-0) (PMID:19675187)
Full text not currently available from Enlighten.
Abstract
Bovine papillonnavirus type 1 is one of the aetiological agents of equine sarcoids. The viral major oncoprotein E5 is expressed in virtually all sarcoids, sarcoid cell lines and in vitro-transformed equine fibroblasts. To ascertain whether E5 behaves in equine cells as it does in bovine cells, we introduced the E5 open reading frame into fetal equine fibroblasts (EqPaIF). As observed in primary bovine fibroblasts (BoPaIF), E5 by itself could not immortalize EqPaIF and an immortalizing gene, such as human telomerase (hTERT/hT), was required for the cells to survive selection. The EqPaIF-hT-1E5 cells were morphologically transformed, elongated with many pseuclopodia and capable of forming foci. Equine major histocompatibility complex class I (MHC I) was inhibited in these cells at least at two levels: transcription of MHC I heavy chain was inhibited and the MHC I complex was retained in the Golgi apparatus and prevented from reaching the cell surface. We conclude that, as in bovine cells and tumours, E5 is a player in the transformation of equine cells and the induction of sarcoids, and a potential major cause of MHC I downregulation and hence poor immune clearance of tumour cells
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Campo, M Saveria and Ellis, Dr S and Gault, Mrs Elizabeth and Nasir, Professor Lubna and Yuan, Dr Zhengqiang and Cortese, Dr Marc and Marchetti, Dr Barbara |
Authors: | Marchetti, B., Gault, E.A., Cortese, M.S., Yuan, Z.Q., Ellis, S.A., Nasir, L., and Campo, M.S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Journal of General Virology |
Publisher: | Society for General Microbiology |
ISSN: | 0022-1317 |
ISSN (Online): | 1465-2099 |
University Staff: Request a correction | Enlighten Editors: Update this record