Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays

Yin, H. , Pattrick, N., Zhang, X.L., Klauke, N., Cordingley, H.C., Haswell, S.J. and Cooper, J.M. (2008) Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays. Analytical Chemistry, 80(1), pp. 179-185. (doi: 10.1021/ac701958z)

Abstract

In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5'-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca2+ flux

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Klauke, Dr Norbert and Yin, Professor Huabing and Cooper, Professor Jonathan
• Authors: Yin, H., Pattrick, N., Zhang, X.L., Klauke, N., Cordingley, H.C., Haswell, S.J., and Cooper, J.M.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Analytical Chemistry
• Journal Abbr.: Anal. chem.
• ISSN: 0003-2700
• ISSN (Online): 1520-6882
• Published Online: 06 December 2007