Watters, A.D. and Bartlett, J.M.S. (2002) Fluorescence in situ hybridization in paraffin tissue sections - Pretreatment protocol. Molecular Biotechnology, 21(3), pp. 217-220.
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Publisher's URL: http://www.humanapress.com/ArticleDetail.pasp?issn=1073-6085&acode=MB:21:3:217
Abstract
Fluorescence in situ hybridization has found wide application in the enumeration of gene and chromosome copy number both in isolated cells and in tissue sections. However, the technique has been less widely used than would be expected in formalin- fixed paraffin processed (archival) tissue. This article describes a method for assessing archival tissue sections, following pretreatment, before applying DNA probes, that gives consistent, reliable results.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Bartlett, Prof John |
| Authors: | Watters, A.D., and Bartlett, J.M.S. |
| Subjects: | R Medicine > RB Pathology |
| College/School: | College of Medical Veterinary and Life Sciences |
| Journal Name: | Molecular Biotechnology |
| ISSN: | 1073-6085 |
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