Vaasa, A., Lust, M., Terrin, A., Uri, A. and Zaccolo, M. (2010) Small-molecule FRET probes for protein kinase activity monitoring in living cells. Biochemical and Biophysical Research Communications, 397(4), pp. 750-755. (doi: 10.1016/j.bbrc.2010.06.026)
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Abstract
In this study, the applicability of fluorescently labeled adenosine analogue-oligoarginine conjugates (ARC-Photo probes) for monitoring of protein kinase A (PKA) activity in living cells was demonstrated. ARC-Photo probes possessing subnanomolar affinity towards the catalytic subunit of PKA (PKAc) and competitive with the regulatory subunit (PKAr), penetrate cell plasma membrane and associate with PKAc fused with yellow fluorescent protein (PKAc-YFP). Detection of inter-molecular F÷rster resonance energy transfer (FRET) efficiency between the fluorophores of the fusion protein and ARC-Photo probe can be used for both the evaluation of non-labeled inhibitors of PKAc and for monitoring of cAMP signaling via detection of changes in the activity of PKA as a cAMP downstream effector.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Zaccolo, Professor Manuela and Terrin, Ms Anna |
| Authors: | Vaasa, A., Lust, M., Terrin, A., Uri, A., and Zaccolo, M. |
| Subjects: | Q Science > QH Natural history > QH345 Biochemistry Q Science > QR Microbiology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
| Journal Name: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| ISSN (Online): | 1090-2104 |
| Published Online: | 10 June 2010 |
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