Oien, K. A. (2003) Serial analysis of gene expression. In: Bartlett, J. M.S. and Stirling, D. (eds.) PCR Protocols. Series: Methods in molecular biology (226). Humana Press: Totowa, NJ, USA, pp. 271-283. ISBN 9780896036420 (doi: 10.1385/1-59259-384-4:271)
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Abstract
Serial analysis of gene expression (SAGE™) is a patented, large-scale mRNA-profiling technology that produces comprehensive, quantitative, and reproducible gene expression profiles (originally described in refs. 1 and 2). Unlike the alternative technologies of differential display and subtractive hybridization, SAGE produces a full catalog of all transcripts, not only differentially expressed genes, and unlike smaller arrays, SAGE needs no assumptions about the genes that are likely to be expressed, thus allowing the identification of novel genes (are among many excellent reviews, see refs. 3, 4, 5). SAGE is based on generating clones of concatenated (linked) short sequence tags derived from mRNA from the target cells or tissue (Fig. 1).
| Item Type: | Book Sections |
|---|---|
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Oien, Professor Karin |
| Authors: | Oien, K. A. |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Publisher: | Humana Press |
| ISBN: | 9780896036420 |
| Related URLs: |
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