Development of a competitive ELISA for detection of Theileria annulata infection

Renneker, S., Kullmann, B., Gerber, S., Dobschanski, J., Bakheit, M.A., Geysen, D., Shiels, B. , Tait, A., Ahmed, J.S. and Seitzer, U. (2008) Development of a competitive ELISA for detection of Theileria annulata infection. Transboundary and Emerging Diseases, 55(5-6), pp. 249-256. (doi:10.1111/j.1865-1682.2008.01036.x) (PMID:18666970)

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Abstract

In previous studies, Theileria annulata surface protein (TaSP) was identified as an immunodominant antigen and successfully used to develop and validate a recombinant-protein-based ELISA for the detection of circulating antibodies in serum of T. annulata-infected animals. In this study, the same antigen was used to develop a competitive ELISA (cELISA) using a monoclonal antibody that was found to bind to TaSP. The cELISA accurately differentiated T. annulata-infected from uninfected animals and demonstrated a satisfactory performance with a calculated sensitivity and specificity of 77.4% and 100%, respectively. Thus the test proved its suitability for the diagnosis of tropical theileriosis and has application for use in serological surveys to monitor the prevalence of the disease or identify carrier animals with high specificity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shiels, Professor Brian and Tait, Professor Andy
Authors: Renneker, S., Kullmann, B., Gerber, S., Dobschanski, J., Bakheit, M.A., Geysen, D., Shiels, B., Tait, A., Ahmed, J.S., and Seitzer, U.
Subjects:Q Science > QR Microbiology
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Transboundary and Emerging Diseases
ISSN:1865-1674
ISSN (Online):0931-4229
Published Online:08 July 2008

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