A modified Nick translation method used with FISH that produces reliable results with archival tissue sections

Watters, A.D., Stacey, M.W. and Bartlett, J.M.S. (2002) A modified Nick translation method used with FISH that produces reliable results with archival tissue sections. Molecular Biotechnology, 20(3), pp. 257-260. (doi: 10.1385/MB:20:3:257)

Abstract

Nick translation is used to label DNA and RNA to produce probes for in situ hybridization and Northern and Southern blotting. Fluorescence in situ hybridization (FISH) is a widely applied technique used to determine chromosomal and genetic anomalies in many biological samples. Initially the technique was applied to metaphase preparations, but the usefulness of detecting genetic anomalies in solid tumors in situ has resulted in the development of modified protocols. Formalin fixed paraffin processed tissue sections present novel challenges when applying FISH; the probes must be small (between 200 and 600 base pairs) and pretreatment is necessary before the probes can be applied to tissue sections, to promote probe access to target DNA. Here we report on a modification of a nick translation method to produce a probe that can reliably be used with FISH in paraffin processed tissue sections.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Bartlett, Prof John
• Authors: Watters, A.D., Stacey, M.W., and Bartlett, J.M.S.
• Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer); R Medicine > RB Pathology
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: Molecular Biotechnology
• ISSN: 1073-6085