Khera, H. K., Valiyaparambil, A., Jagannath, D. K., Viswanath, V. K., Kumar, N., Shukla, J. P., Pradhan, S. and Lakshminarasimhan, A. (2024) Nano-differential scanning fluorimetry as a tool for the assessment of refolded antibody fragments: a case study for anti-Pfs25 single-chain antibodies. Biochemical Engineering Journal, 206, 109287. (doi: 10.1016/j.bej.2024.109287)
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Abstract
Differential Scanning Fluorimetry (DSF) is a valuable and versatile tool in the field of protein and antibody stability studies, providing valuable insights into their thermal stability, and aiding in the optimization of experimental conditions for various applications. Antibody fragments provide armamentarium for researchers and practitioners across various fields, enabling advancements in medicine, diagnostics, research, and industrial applications. In the present study, single-chain antibodies derived from mAb 4B7 and mAb 1245 were expressed in E. coli, refolded, and checked for their binding with the antigen - Pfs25 (transmission-blocking malaria vaccine candidate). We used nano-DSF as a valuable tool to assess thermal stability, consequently aiding in predicting the correct folding of single-chain antibodies.. Employing nano-DSF as a checkpoint enables the determination of whether to proceed with functional and binding studies on the refolded single-chain antibodies.
| Item Type: | Articles |
|---|---|
| Additional Information: | The authors would like to thank Tata trusts for funding this study. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Pradhan, Dr Sabyasachi |
| Creator Roles: | |
| Authors: | Khera, H. K., Valiyaparambil, A., Jagannath, D. K., Viswanath, V. K., Kumar, N., Shukla, J. P., Pradhan, S., and Lakshminarasimhan, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Biochemical Engineering Journal |
| Publisher: | Elsevier |
| ISSN: | 1369-703X |
| ISSN (Online): | 1873-295X |
| Published Online: | 02 March 2024 |
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