Namuwulya, P. et al. (2024) Viruses associated with measles-like illnesses in Uganda. Journal of Infection, (doi: 10.1016/j.jinf.2024.106148) (PMID:38588959) (In Press)
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Abstract
Objectives: In this study, we investigated the causes of measles-like illnesses (MLI) in the Uganda national surveillance programme in order to inform diagnostic assay selection and vaccination strategies. Methods: We used metagenomic next-generation sequencing (M-NGS) on the Illumina platform to identify viruses associated with MLI (defined as fever and rash in the presence of either cough, coryza or conjunctivitis) in patient samples that had tested IgM negative for measles between 2010 and 2019. Results: Viral genomes were identified in 87/271 (32%) of samples, of which 44/271 (16%) contained 12 known viral pathogens. Expected viruses included rubella, human parvovirus B19, Epstein Barr virus, human herpesvirus 6B, human cytomegalovirus, varicella zoster virus and measles virus (detected within the seronegative window-period of infection) and the blood-borne hepatitis B virus. We also detected Saffold virus, human parvovirus type 4, the human adenovirus C2 and vaccine-associated poliovirus type 1. Conclusions: The study highlights the presence of undiagnosed viruses causing MLI in Uganda, including vaccine-preventable illnesses. NGS can be used to monitor common viral infections at a population level, especially in regions where such infections are prevalent, including low and middle income countries to guide vaccination policy and optimize diagnostic assays.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by the Medical Research Council-Centre for Virus Research (CVR) University of Glasgow (MC_UU_1201412), Wellcome (102789/Z/13/Z), a Royal Society of Tropical Medicine and Hygiene (RSTMH) small grant 2018 and GeMVi: Application of Genomics and Modelling to the Control of Virus Pathogens -National Institute for Health and Care Research (NIHR) (project reference 17/63/82) using UK aid from the UK Government to support global health research. |
| Keywords: | Measles-like illness, serum, metagenomic next-generation sequencing, viruses, Uganda. |
| Status: | In Press |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Da Silva Filipe, Dr Ana and Thomson, Professor Emma and Niebel, Dr Marc and Ssekagiri, Mr Alfred and Tong, Dr Lily and Namuwulya, Ms Prossy and Davis, Dr Chris and Ashraf, Dr Shirin |
| Authors: | Namuwulya, P., Ashraf, S., Niebel, M., Ssekagiri, A., Tushabe, P., Kakooza, P., Tong, L., Bukenya, H., Jerome, H., Davis, C., Birungi, M., Turyahabwe, I., Mugaga, A., Eliku, J. P., Francis, A., Nakabazzi, L., Nsubuga, F., Katushabe, E., Kisakye, A., Ampeire, I., Nanteza, A., Kaleebu, P., Bakamutumaho, B., Nsamba, P., Kazibwe, A., da Silva Filipe, A., Tweyongyere, R., Bwogi, J., and Thomson, E. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of Infection |
| Publisher: | Elsevier |
| ISSN: | 0163-4453 |
| ISSN (Online): | 1532-2742 |
| Published Online: | 06 April 2024 |
| Copyright Holders: | Copyright © 2024 Elsevier Ltd on behalf of The British Infection Association |
| First Published: | First published in Journal of Infection 2024 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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