Sénéchal, F., Robinson, S., Van Schaik, E., Trévisan, M., Saxena, P. , Reinhardt, D. and Fankhauser, C. (2024) Pectin methylesterification modulates cell wall properties to promote neighbour proximity-induced hypocotyl growth. Plant Direct, 8(4), e584. (doi: 10.1002/pld3.584)
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Abstract
Plants growing with neighbors compete for light and consequently increase the growth of their vegetative organs to enhance access to sunlight. This response, called shade avoidance syndrome (SAS), involves photoreceptors such as phytochromes as well as phytochrome interacting factors (PIFs), which regulate the expression of growth-mediating genes. Numerous cell wall-related genes belong to the putative targets of PIFs, and the importance of cell wall modifications for enabling growth was extensively shown in developmental models such as dark-grown hypocotyl. However, the contribution of the cell wall in the growth of de-etiolated seedlings regulated by shade cues remains poorly established. Through analyses of mechanical and biochemical properties of the cell wall coupled with transcriptomic analysis of cell wall-related genes from previously published data, we provide evidence suggesting that cell wall modifications are important for neighbor proximity-induced elongation. Further analysis using loss-of-function mutants impaired in the synthesis and remodeling of the main cell wall polymers corroborated this. We focused on the cgr2cgr3 double mutant that is defective in methylesterification of homogalacturonan (HG)-type pectins. By following hypocotyl growth kinetically and spatially and analyzing the mechanical and biochemical properties of cell walls, we found that methylesterification of HG-type pectins was required to enable global cell wall modifications underlying neighbor proximity-induced hypocotyl growth. Collectively, our work suggests that plant competition for light induces changes in the expression of numerous cell wall genes to enable modifications in biochemical and mechanical properties of cell walls that contribute to neighbor proximity-induced growth.
| Item Type: | Articles |
|---|---|
| Additional Information: | Funding information: This work was supported by the grant “Plant Growth 2 in a Changing Environment” funded by the Swiss initiative in systems biology (SystemX.ch) to Cris Kuhlemeier (Bern), Didier Reinhardt (Fribourg), and Christian Fankhauser (Lausanne). Work in the Fankhauser lab was supported by the University of Lausanne and the Swiss National Science Foundation (grant 310030B_179558). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Saxena, Dr Prashant |
| Creator Roles: | |
| Authors: | Sénéchal, F., Robinson, S., Van Schaik, E., Trévisan, M., Saxena, P., Reinhardt, D., and Fankhauser, C. |
| College/School: | College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | Plant Direct |
| Publisher: | Wiley for the Society for Experimental Biology and the American Society of Plant Biologists |
| ISSN: | 2475-4455 |
| ISSN (Online): | 2475-4455 |
| Published Online: | 21 April 2024 |
| Copyright Holders: | Copyright © 2024 The Authors |
| First Published: | First published in Plant Direct 8(4):e584 |
| Publisher Policy: | Reproduced under a Creative Commons license |
| Related URLs: |
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