Rimbi, P. T., O'Boyle, N., Douce, G. R. , Pizza, M., Rosini, R. and Roe, A. J. (2024) Enhancing a multi-purpose artificial urine for culture and gene expression studies of uropathogenic Escherichia coli strains. Journal of Applied Microbiology, lxae067. (doi: 10.1093/jambio/lxae067) (PMID:38486355) (Early Online Publication)
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Abstract
Aims: The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains. Methods and results: We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU. Transcriptomic analysis of UPEC strains cultured in enhanced artificial urine (enhanced AU) and PHU showed that the gene expression profiles are similar, with less than 7% of genes differentially expressed between the two conditions. Conclusions: Enhancing an MP-AU with metabolites identified in PHU allows the enhanced AU to be used as a substitute for the culture and in vitro gene expression studies of UPEC strains.
| Item Type: | Articles |
|---|---|
| Additional Information: | PTR was funded by a University of Glasgow PhD studentship in collaboration with GSK. NOB was supported by the Biotechnology and Biological Sciences Research Council [BB/R006539/1]. GRD and AJR were supported by the Biotechnology and Biological Sciences Research Council [BB/W015781/1]. AJR was also supported by the Medical Research Council [MR/V011499/1]. |
| Keywords: | artificial urine, uropathogen, E. coli, transcriptome. |
| Status: | Early Online Publication |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Douce, Dr Gillian and Roe, Professor Andrew and Rimbi, Patricia |
| Creator Roles: | Rimbi, P.Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editing Douce, G.Conceptualization, Funding acquisition, Methodology, Project administration, Supervision Roe, A.Conceptualization, Methodology, Project administration, Supervision, Writing – review and editing |
| Authors: | Rimbi, P. T., O'Boyle, N., Douce, G. R., Pizza, M., Rosini, R., and Roe, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Applied Microbiology |
| Publisher: | Oxford University Press |
| ISSN: | 1364-5072 |
| ISSN (Online): | 1365-2672 |
| Published Online: | 14 March 2024 |
| Copyright Holders: | Copyright © 2024 The Author(s) |
| First Published: | First published in Journal of Applied Microbiology 2024 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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