Kane, N. M., Meloni, M., Spencer, H. L., Craig, M. A., Strehl, R., Milligan, G., Houslay, M. D., Mountford, J. C., Emanueli, C. and Baker, A. H. (2010) Derivation of endothelial cells from human embryonic stem cells by directed differentiation: analysis of microRNA and angiogenesis in vitro and in vivo. Arteriosclerosis, Thrombosis, and Vascular Biology, 30(7), pp. 1389-1397. (doi: 10.1161/ATVBAHA.110.204800)
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Abstract
<b>Objective</b>: To develop an embryoid body-free directed differentiation protocol for the rapid generation of functional vascular endothelial cells derived from human embryonic stem cells (hESCs) and to assess the system for microRNA regulation and angiogenesis.<p></p> <b>Methods and Results</b>: The production of defined cell lineages from hESCs is a critical requirement for evaluating their potential in regenerative medicine. We developed a feeder- and serum-free protocol. Directed endothelial differentiation of hESCs revealed rapid loss of pluripotency markers and progressive induction of mRNA and protein expression of vascular markers (including CD31 and vascular endothelial [VE]-cadherin) and angiogenic growth factors (including vascular endothelial growth factor), increased expression of angiogenesis-associated microRNAs (including miR-126 and miR-210), and induction of endothelial cell morphological features. In vitro, differentiated cells produced nitric oxide, migrated across a wound, and formed tubular structures in both the absence and the presence of 3D matrices (Matrigel). In vivo, we showed that cells that differentiated for 10 days before implantation were efficient at the induction of therapeutic neovascularization and that hESC-derived cells were incorporated into the blood-perfused vasculature of recipient mice.<p></p> <b>Conclusion</b>: The directed differentiation of hESCs is efficient and effective for the differentiation of functional endothelial cells from hESCs.<p></p>
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Houslay, Professor Miles and Baker, Professor Andrew and Spencer, Miss Helen and Kane, Dr Nicole and Mountford, Dr Joanne and Milligan, Professor Graeme and Craig, Dr Margaret and Meloni, Dr Marco |
| Authors: | Kane, N. M., Meloni, M., Spencer, H. L., Craig, M. A., Strehl, R., Milligan, G., Houslay, M. D., Mountford, J. C., Emanueli, C., and Baker, A. H. |
| Subjects: | Q Science > QR Microbiology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
| Journal Name: | Arteriosclerosis, Thrombosis, and Vascular Biology |
| Publisher: | American Heart Association |
| ISSN: | 1079-5642 |
| ISSN (Online): | 1524-4636 |
| Published Online: | 29 April 2010 |
| Copyright Holders: | Copyright © 2010 American Heart Association |
| First Published: | First published in Arteriosclerosis, Thrombosis, and Vascular Biology 30(7):1389-1397 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
| Related URLs: |
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