Evaluation of the DN-Mini (miniaturized double net) trap for sampling host-seeking Anopheles mosquitoes in malaria-endemic villages of southern Tanzania.

Limwagu, A. J., Msugupakulya, B. J., Kilalangongono, M. M., Mwalugelo, Y. A., Okumu, F. O. , Lyimo, I. N. and Ngasala, B. E. (2024) Evaluation of the DN-Mini (miniaturized double net) trap for sampling host-seeking Anopheles mosquitoes in malaria-endemic villages of southern Tanzania. PLoS ONE, 19(2), e0294192. (doi: 10.1371/journal.pone.0294192) (PMID:38354118) (PMCID:PMC10866531)

Abstract

Background: Surveillance of malaria vectors is crucial for assessing the transmission risk and impact of control measures. Human landing catches (HLC) directly estimate the biting rates but raise ethical concerns due to the exposure of volunteers to mosquito-borne pathogens. A common alternative is the CDC-light trap, which is effective for catching host-seeking mosquitoes indoors but not outdoors. New, exposure-free methods are needed for sampling mosquitoes indoors and outdoors in ways that reflect their natural risk profiles. The aim of this study was therefore to evaluate the efficacy of the miniaturized double net trap (DN-Mini) for sampling host-seeking mosquitoes in south-eastern Tanzania, where malaria transmission is dominated by Anopheles funestus. Methods: Adult mosquitoes were collected from 222 randomly selected houses across three villages (74 per village) in Ulanga district, south-eastern Tanzania, using the DN-Mini traps, CDC-Light traps, and Prokopack aspirators. First, we compared CDC-light and DN-Mini traps for collecting indoor host-seeking mosquitoes, while Prokopack aspirators were used for indoor-resting mosquitoes. Second, we deployed the DN-Mini and Prokopack aspirators to collect host-seeking and resting mosquitoes indoors and outdoors. Generalized linear mixed models (GLMM) with a negative binomial distribution were used to compare the effectiveness of the traps for catching different mosquito species. Results: The DN-Mini was 1.53 times more efficient in collecting An. funestus indoors (RR = 1.53, 95% CI: 1.190–1.98) compared to the CDC-Light trap. However, for Anopheles arabiensis, the DN-Mini caught only 0.32 times as many mosquitoes indoors as the CDC-Light traps (RR = 0.32, 95% CI: 0.183–0.567). Both An. funestus and An. arabiensis were found to be more abundant indoors than outdoors when collected using the DN-Mini trap. Similarly, the Prokopack aspirator was greater indoors than outdoors for both An. funestus and An. arabiensis. Conclusion: The DN-Mini outperformed the CDC-light trap in sampling the dominant malaria vector, An. funestus species, but was less effective in capturing An. arabiensis, and for both vector species, the biting risk was greater indoors than outdoors when measured using the DN-Mini trap. These findings highlight the importance of selecting appropriate trapping methods based on mosquito species and behaviors.

• Item Type: Articles
• Additional Information: Funding: his study was funded by the Bill and Melinda Gates Foundation, awarded through the Pan-African Mosquito Control Association (PAMCA) (Grant No. OPP 1214408, Ifakara Health Institute).
• Keywords: Animals, Humans, Anopheles, Malaria, CD40 Ligand, Entomology, Mosquito Control, Adult, Tanzania, Mosquito Vectors
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Okumu, Professor Fredros

Creator Roles

Okumu, F.: Conceptualization, Funding acquisition, Methodology, Resources, Writing – review and editing

• Authors: Limwagu, A. J., Msugupakulya, B. J., Kilalangongono, M. M., Mwalugelo, Y. A., Okumu, F. O., Lyimo, I. N., and Ngasala, B. E.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: PLoS ONE
• Publisher: Public Library of Science
• ISSN: 1932-6203
• ISSN (Online): 1932-6203
• Copyright Holders: Copyright: © 2024 Limwagu et al.
• First Published: First published in PLoS ONE 19(2): e0294192
• Publisher Policy: Reproduced under a Creative Commons licence