The deubiquitylase USP31 controls the Chromosomal Passenger Complex and spindle dynamics

Bertsoulaki, E., Glover, H. L. , Gomes-Neto, J. I., Pizer, B., Maiato, H., Urbé, S. and Clague, M. J. (2022) The deubiquitylase USP31 controls the Chromosomal Passenger Complex and spindle dynamics. bioRxiv, (doi: 10.1101/2022.08.17.504168) (Unpublished)

Abstract

We have identified USP31 as a microtubule and centrosome associated deubiquitylase, depletion of which leads to an increase in individual cell mass and defective proliferation. We have examined its dynamics and impact during mitosis. GFP-USP31 associates with the mitotic and central spindles, its levels are increased 2-3-fold in prometaphase compared to asynchronous cells and it is dynamically phosphorylated in a CDK1 dependent manner. We find that USP31 depleted cells display altered spindle morphology and chromosomal segregation errors, whilst stable expression of a catalytically inactive form of USP31 leads to polyploidy. At prometaphase, levels of multiple CPC components are destabilised, most prominently INCENP. Under anaphase conditions, depletion of USP31 impairs the translocation of both endogenous and ectopically expressed INCENP to the spindle mid-zone, whilst expression of catalytically inactive USP31 results in multiple ectopic cleavage furrows. In summary, our data indicate a multifaceted regulatory role for USP31 during mitosis, with a profound impact on chromosomal passenger complex abundance, dynamics and function.

• Item Type: Articles
• Status: Unpublished
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Glover, Ms Hannah
• Authors: Bertsoulaki, E., Glover, H. L., Gomes-Neto, J. I., Pizer, B., Maiato, H., Urbé, S., and Clague, M. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: bioRxiv
• Copyright Holders: Copyright © 2022 The Author(s)/Funder
• Publisher Policy: Reproduced under a creative commons licence

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