Wu, Y.‐L., Zhu, A.‐Q., Zhou, X.‐T., Zhang, K.‐W., Yuan, X.‐J., Yuan, M., He, J., Pineda, M. A. and Li, K.‐P. (2024) A novel ultrafiltrate extract of propolis exerts anti‐inflammatory activity through metabolic rewiring. Chemistry and Biodiversity, 21(3), e202301315. (doi: 10.1002/cbdv.202301315) (PMID:38189169)
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Abstract
Thousands of years ago, humans started to use propolis because of its medicinal properties, and modern science has successfully identified several bioactive molecules within this resinous bee product. However, a natural propolis extract which has been removed the adhesive glue and preserved propolis bioactive compounds is urgently needed to maximise the therapeutic opportunities. In this study, a novel ultrafiltrate fraction from Brazilian green propolis, termed P30K, was demonstrated with anti-inflammatory properties, both in vitro and in vivo. Total flavonoids and total phenolic acids content in P30K were 244.6 mg/g and 275.8 mg/g respectively, while the IC50 value of inhibition of cyclooxygenase-2 (COX-2) was 8.30 μg/mL. The anti-inflammatory activity of P30K was furtherly corroborated in experimental models of lipopolysaccharides (LPS)-induced acute liver and lung injury. Mechanistically, integrated GC−MS and LC−MS based serum metabolomics analysis revealed that P30K modulated citrate cycle (TCA), pyruvate, glyoxylate and dicarboxylate metabolism pathways to inhibit secretion of pro-inflammatory cytokines. Results of network pharmacology and molecular docking suggested that P30K targeted catechol-O-methyltransferases (COMT), 11β-hydroxysteroid dehydrogenases (HSD11B1), and monoamine oxidases (MAOA and MAOB) to promote cellular metabolomic rewiring. Collectively, our work reveals P30K as an efficient therapeutic agent against inflammatory conditions and its efficacy is related to metabolic rewiring.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported partly by Guangdong Natural Science Foundation (2021A1515012105, 2023A1515011128) and Under-graduate Innovation and Entrepreneurship Training Program of Guangdong Pharmaceutical University (202010573031). |
| Keywords: | Molecular Biology, Molecular Medicine, General Chemistry, Biochemistry, General Medicine, Bioengineering |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Pineda, Dr Miguel |
| Authors: | Wu, Y.‐L., Zhu, A.‐Q., Zhou, X.‐T., Zhang, K.‐W., Yuan, X.‐J., Yuan, M., He, J., Pineda, M. A., and Li, K.‐P. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Chemistry and Biodiversity |
| Publisher: | Wiley |
| ISSN: | 1612-1872 |
| ISSN (Online): | 1612-1880 |
| Published Online: | 29 January 2024 |
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