Eutirucallin: A lectin with antitumor and antimicrobial properties

Palharini, J. G., Richter, A. C., Ferreira Silva, M., Ferreira, F. B., Pirovani, C. P., Naves, K. S.C., Goulart, V. A., Mineo, T. W.P., Silva, M. J.B. and Santiago, F. M. (2017) Eutirucallin: A lectin with antitumor and antimicrobial properties. Frontiers in Cellular and Infection Microbiology, 7, 136. (doi: 10.3389/fcimb.2017.00136) (PMID:28487845) (PMCID:PMC5403948)

Abstract

Eutirucallin is a lectin isolated from the latex of Euphorbia tirucalli, a plant known for its medical properties. The present study explores various characteristics of Eutirucallin including stability, cytotoxicity against tumor cells, antimicrobial and antiparasitic activities. Eutirucallin was stable from 2 to 40 days at 4°C, maintained hemagglutinating activity within a restricted range, and showed optimal activity at pH 7.0–8.0. Eutirucallin presented antiproliferative activity for HeLa, PC3, MDA-MB-231, and MCF-7 tumor cells but was not cytotoxic for non-tumorigenic cells such as macrophages and fibroblasts. Eutirucallin inhibited the Ehrlich ascites carcinoma in vivo and it was also observed that Eutirucallin inhibited 62.5% of Escherichia coli growth. Also, Eutirucallin showed to be effective when tested directly against Toxoplasma gondii infection in vitro. Therefore, this study sheds perspectives for pharmacological applications of Eutirucallin.

• Item Type: Articles
• Additional Information: This work was supported by Brazilian Funding Agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; AUX-PE-PARASITOLOGIA 1348/2011), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 309011/2013-2), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG; CDS-RED-00013-14, CVZ-PPM-00784-15).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ferreira Silva, Miss Mariana
• Authors: Palharini, J. G., Richter, A. C., Ferreira Silva, M., Ferreira, F. B., Pirovani, C. P., Naves, K. S.C., Goulart, V. A., Mineo, T. W.P., Silva, M. J.B., and Santiago, F. M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Frontiers in Cellular and Infection Microbiology
• Publisher: Frontiers Media
• ISSN: 2235-2988
• ISSN (Online): 2235-2988
• Copyright Holders: Copyright © 2017 2017 Palharini, Richter, Silva, Ferreira, Pirovani, Naves, Goulart, Mineo, Silva and Santiago
• First Published: First published in Frontiers in Cellular and Infection Microbiology 7:136
• Publisher Policy: Reproduced under a creative commons licence