Shaw, A. M. and Gammage, P. A. (2023) Coupling Differential Centrifugation with Exonuclease Treatment and Size Exclusion Chromatography (DIFSEC) for purification of mtDNA from mammalian cells. In: Mitochondrial DNA: Methods and Protocols. Series: Methods in molecular biology (2615). Springer: New York, pp. 31-40. ISBN 9781071629215
Full text not currently available from Enlighten.
Publisher's URL: https://link.springer.com/protocol/10.1007/978-1-0716-2922-2_3
Abstract
Direct analysis of mtDNA using PCR-free methods is limited by the presence of persistent, contaminating nucleic acids originating from the nuclear genome, even following stringent mitochondrial isolations. Here we describe a method developed in our laboratory that couples existing, commercially available mtDNA isolation protocols with exonuclease treatment and size exclusion chromatography (DIFSEC). This protocol produces highly enriched mtDNA extracts from small-scale cell culture, with near-undetectable nuclear DNA contamination.
| Item Type: | Book Sections |
|---|---|
| Additional Information: | eISBN: 9781071629222 |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Gammage, Dr Payam |
| Authors: | Shaw, A. M., and Gammage, P. A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Research Group: | Gammage |
| Publisher: | Springer |
| ISBN: | 9781071629215 |
| Related URLs: |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details